Drought response identified in potential biofuel plant
Drought resistance is the key to large-scale production of Jatropha, a potential biofuel plant -- and an international group of scientists has identified the first step toward engineering a hardier variety.
Jatropha has seeds with high oil content. But the oil's potential as a biofuel is limited because, for large-scale production, this shrub-like plant needs the same amount of care and resources as crop plants.
"It is thought that Jatropha's future lies in further improvement of Jatropha for large-scale production on marginal, non-food croplands through breeding and/or biotechnology," said John E. Carlson, professor of molecular genetics at Penn State. "The more that is known about the genetic basis of Jatropha's key attributes such as drought tolerance, the more readily Jatropha improvement will progress."
According to Carlson, Jatropha currently grows best in tropical countries and is already being cultivated as a biofuel on a small scale in India, Southeast Asia and Africa. Breeding a strain that could do well in arid, barren conditions could enable mass cultivation, but large-scale production may still be decades away.
Researchers looked at a little known gene -- JcPIP1 -- because a similar gene in the model plant Arabidopsis is known to play a role in drought response. They also examined JcPIP2, a potential drought response gene in Jatropha identified in 2007 by researchers at Sichuan University. They reported their findings today (July 15) in the Journal of Plant Physiology.
The JcPIP genes code for membrane channels called aquaporins, which are responsible for transporting and balancing water throughout the plant, though exactly how each gene affects aquaporin behavior under environmental stress remains unclear. However, researchers have found that JcPIP1 and JcPIP2 are expressed at different times during a stressful situation, which hints at what roles they play in response and recovery.
By growing unmodified Jatropha samples in conditions simulating high soil salinity and low water availability, the researchers showed that Jatropha was normally more vulnerable and slower to recover from high salinity than from drought conditions.
Using a tobacco mosaic virus to transiently transform Jatropha, the researchers created plants in which JcPIP2 or JcPIP1 was temporarily disabled. They subjected the modified samples to six days of stress and six days of recovery. To gauge the plants' stress responses, they noted physical changes and measured root damage, leaf growth, electrolyte leakage in the leaves, and sap flow and volume.